Updated Tissue Collection Protocol

As the mycoflora project continues to progress, we will continue working to streamline the protocols being used by participating projects and our partner labs. One of the first significant changes in our protocols is a shift to collecting tissue from dried specimens in empty microcentrifuge tubes. Up until this time, we accepted tissue in 2.0 mL microcentrifuge tubes that contained a buffer solution. These tubes allowed the tissue to be stored from fresh specimens. We are now shifting exclusively to 1.5 mL microcentrifuge tubes without a buffer solution. This change requires that specimens be dried before the tissue is extracted and placed in a tube

A second requirement is that a small portion of the specimen be sent along with the tissue collection tubes. Along with your tube order, you will also be sent small plastic bags. Please include a small portion of each collection in these bags, label them with the collection number, and send them along with the tubes to the sequencing facility. The main portion of the collection will still be sent to your partner herbarium. These secondary smaller collections in the plastic bags, commonly called a "split" of the collection, should consist of a small individual mushroom from collections with multiple specimens, or for single specimens, a piece of the primary collection about the size of a quarter. For small samples, it is fine to send a smaller portion. If splitting the collection is not possible without destroying or significantly reducing the primary collection, retain the primary collection in-tact and do not send a split of that sample.

One additional note: Projects that are utilizing Duke University in North Carolina as their primary herbarium can send the specimens in-tact, without creating the split. 

These changes to the protocols will take effect immediately. Awardees of the fall 2018 awards will be sent microcentrifuge tubes without a buffer solution.

Tissue Collection Protocol at Mycoflora.org

If you have any questions about these changes, please email This email address is being protected from spambots. You need JavaScript enabled to view it.

About North American Mycoflora Project

NAMP is a 501(c)(3) non-profit organization. We are working towards a single goal - the development of the first comprehensive mycoflora of North America. This project is a consortium of citizen scientists and professional mycologists performing a biological survey of all the macrofungi that occur in North America.

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